It is our great pleasure to invite you to the eighth hands-on training school of "Basics to work with extracellular vesicles" jointly organized by MOVE (Mobility for Vesicles Research in Europe) , OH-Boost, University of Tartu, Baltic Society of extracellular vesicles, University of Helsinki and University of Milan to be held from 2nd-4th of October 2025 in Tartu, Estonia.
Instructors
Program
Methods of EV Isolation
09:00 - 09:20 |
Welcoming and Introduction |
Alireza Fazeli |
09:20 - 10:05 |
Lecture 1 |
Subhashini Muhandiram |
A guide to working with extracellular vesicles: minimal experimental and reporting requirements |
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10:05 - 10:25 |
Tea & Coffee break |
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10:25 - 11:10 |
Lecture 2 |
Qurat Ul Ain Reshi |
Methods for EV purification |
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11:10 - 12:10 |
Student 3-minute presentations (Part 1) |
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12:10 - 13:10 |
Lunch |
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13:10 - 15:10 |
Workshop 1 |
Getnet Midekessa |
Introduction to Tangential Flow Filtration (TFF) |
Gayandi Ekanayake |
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15:10 - 15:30 |
Tea & Coffee break |
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15:30 - 17:00 |
Workshop 2 |
Signe Parts |
Size exclusion chromatography using manually packed chromatography column |
Madhusha Prasadani |
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EV characterization and validation methods
09:00 - 09:40 |
Lecture 3 |
Sascha Raschke |
Introduction to Zeta View Nano particle tracking analyser |
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09:40 - 10:20 |
Lecture 4 |
Sergei Kopanchuk |
Advanced microscopy to work with extracellular vesicles |
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10:20 - 10:40 |
Tea & Coffee break |
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10:40 - 11:10 |
Student 3-minute presentations (Part 2) |
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11:10 - 12:20 |
Workshop 3 |
Chanaka Premathilaka |
EV size and concentration measurements with NTA |
Omid Mousavi |
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12:20 - 13:20 |
Lunch |
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13:20 - 14:20 |
Workshop 4 |
Chanaka Premathilaka |
Fluoroscence NTA |
Omid Mousavi |
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14:20 - 15:20 |
Workshop 5 |
Chanaka Premathilaka |
EV zeta-potential measurements with NTA |
Omid Mousavi |
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15:20 - 15:40 |
Tea & Coffee break |
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15:40 - 16:45 |
Workshop 6 |
Madhusha Prasadini |
NTA data analysis |
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16:45 - 17:50 |
Workshop 7 |
Sergei Kopanchuk |
Advanced microscopy to work with extracellular vesicles |
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19:00 onwards |
Dinner |
Only with invitation |
EV validation methods and further applications
09:00 - 10:00 |
Lecture 5 |
Aneta Andronowska |
Transmission Electron Microscopy (TEM) |
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10:00 - 10:50 |
Lecture 6 (Virtual) |
Harinda Rajapaksha |
Introduction to Lipidomics |
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10:50 - 11:10 |
Tea & Coffee break |
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11:10 - 11:45 |
Workshop 8 |
Harinda Rajapaksha |
Introduction to EV Proteomics |
Subhashini Muhandiram Suranga Kodithuwakku |
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11:45 - 12:20 |
Workshop 9 (Part 1) |
Kasun Godakumara |
EV RNA cargo analysis and RNA extraction from EVs |
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12:20 - 13:20 |
Lunch |
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13:20 - 15:00 |
Workshop 9 (Part 2) |
Kasun Godakumara |
EV RNA cargo analysis and RNA extraction from EVs |
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15:00 - 15:20 |
Tea Break |
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15:20 - 17:00 |
Workshop 10 |
Kasun Godakumara |
EV RNA quality of and integrity analysis |
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17:00-17:30 |
Final student Evaluation |
Evaluation Committee |
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