Researcher, Lithuanian University of Health Sciences, Kaunas, Lithuania
Emilija Šipailaitė work at Lithuanian University of Health Sciences. She has a Pharmacy Master’s degree. She has carried out research in the cell culture laboratory for the last 3 years. During that period, she had a chance to work with many different cell cultures: human skin cells, stem cells and various cancerous cells. She has experience working with exosomes from stem cells and NT2 Parkinson’s disease cybrids. Also, She took part in education programme “Basics to Work With Extracellular Vesicles” in the Institute of Biomedicine and Traslational medicine (MVBS.TK.013), 26 hours (1 ECTS). In addition, in the summer of 2021 I had an ERASMUS+ traineeship “Exosome signaling in bidirectional Microglia - Neuron communication” in University of Coimbra, Portugal. Recently, her field of interest is plant extracellular vesicles of plants and their effect to human cells.
Investigation of cucumber extracellular vesicles and their bioactivity on
human skin cells
Plant-derived extracellular vesicles (EVs) are a novel research subject because of their potential to be used in improving and functionalization of biopharmaceutical delivery. EVs are attractive delivery systems due to their ability to increase stability, solubility, and bioavailability of active agents of the plant. This study aimed to evaluate cucumber EV bioactivity on human skin cells. Fluorescence microscopy confirmed the internalization of plant-derived EVs into the human skin cells after 24 hours. PrestoBlue™ metabolic cell assay revealed that EVs from cucumber fruits significantly increase proliferation of keratinocytes (HaCaT) and fibroblasts (HDF) after 48 hours. Moreover, the EVs shortened wound healing time in in vitro skin model. Therefore, EVs isolated from cucumber can be applied for development of regenerative cosmeceutical and medicinal products.