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  • University of Life Sciences
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5-7th of September, Ravila 14B, Tartu, Estonia
RESEARCH SCHOOL 2023
BASICS TO WORK WITH
​EXTRACELLULAR VESICLES
It is our great pleasure to invite you to second hands-on training school of "AU.873 Basics to work with extracellular vesicles (1ECTS)" jointly organized by COMBIVET ERA Chair of Comparative Medicine in the Institute of Veterinary Medicine and Animal Sciences of the Estonian University of Life Sciences, University of Tartu, Baltic Society of extracellular vesicles, University of Helsinki and University of Milan to be held from 5-7th of September 2023 in Tartu, Estonia.
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TRAVEL INFORMATION
Instructors
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KASUN GODAKUMARA
Estonian University of Life Sciences
Biography
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GETNET MIDEKESSA
Estonian University of Life Sciences
Biography
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QURAT UL AIN RESHI
Estonian University of Life Sciences
Biography
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CHANAKA PREMATHILAKA
Estonian University of Life Sciences
Biography
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DULMINI N. SAPUGAHAWATTE
Estonian University of Life Sciences
Biography
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SEYED OMID
REZA MOUSAVI
​
Estonian University of Life Sciences
Biography
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SUBHASHINI MUHANDIRAM
​Estonian University of Life Sciences
Biography
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GAYANDI EKANAYAKA
​​Estonian University of Life Sciences
Biography
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MADHUSHA PRASADANI
​Estonian University of Life Sciences
Biography
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SIGNE
PARTS
University of Tartu
​

BIOGRAPHY
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JOHANNA
​PIIBOR
​Estonian University of Life Sciences
Biography
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SERGEI KOPANCHUK
University of Tartu​
​
Biography
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OLAVI
​REINSALU​
University of Tartu​
​
Biography
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ANETA ANDRONOWSKA
Polish Academy of Sciences
Biography
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MINNA HOLOPAINEN
University of Helsinki
​
Biography
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SASHA
RASCHKE
University of Düsseldorf
Biography
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CAIUS
​MILLER
University of Tartu​
​
Biography
Program
  • 5th of September
  • 6th of September
  • 7th of September
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>

Methods of EV Isolation

​09:00 - 09:20
​Welcoming and Introduction
​ Alireza Fazeli
0​9:20 - 10:05
Lecture 1
Kasun Godakumara
 
​A guide to working with extracellular vesicles: minimal experimental and reporting requirements
 
​10:05 - 10:25
Tea & Coffee break
 
​10:25 - 11:10
Lecture 2
Qurat Ul Ain Reshi
 
​Methods for EV purification
 
11:10 - 12:10
Student 3-minute presentations 
 
12:10 - 13:10
Lunch
 
​13:10 - 15:10
Workshop 1
​​Getnet Midekessa
 
​Introduction to Tangential Flow Filtration (TFF)
​
​Gayandi Ekanayake
​15:10 - 15:30
​Tea & Coffee break
 
​15:30 - 17:00
Workshop 2
Qurat Ul Ain Reshi 
 
​Size exclusion chromatography using manually packed chromatography column
​Madhusha Prasadani
 
 
Signe Parts
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

EV characterization and validation methods

0​9:00 - 09:40
Lecture 3
Sascha Raschke 
 
​Introduction to Zeta view Nano particle tracking analyser
 
0​9:40 - 10:20
Lecture 4
Olavi Reinsalu
 
​Flow Cytometric analysis of EVs
 
​10:20 - 10:40
Tea & Coffee break
  
​10:40 - 11:10
Student 3-minute presentations 
 
​11:10 - 12:20
Workshop 3
Getnet Midekessa
 
​EV size and concentration measurements with NTA
​
Chanaka Premathilaka
​Omid Mousavi  
​12:20 - 13:20
Lunch
 
13:20 - 14:20
Workshop 4
Getnet Midekessa
 
​Fluoroscence NTA​
​
Chanaka Premathilaka
Omid Mousavi 
​14:20 - 15:20
Workshop 5
Getnet Midekessa
 
EV zeta-potential measurements with NTA
​
Chanaka Premathilaka
Omid Mousavi
​15:20 - 15:40
Tea & Coffee break
 
​15:40 - 17:10
Workshop 6
Johanna Piibor
 
​NTA data analysis  
 
​17:10 - 17:50
Workshop 7
Olavi Reinsalu
 
​Flow Cytometric analysis of EVs
Dulmini Nanayakkara
​19:00 onwards
Dinner
Only with invitation

EV validation methods and further applications

​09:00 - 09:40
Lecture 5
Aneta Andronowska
 
​Transmission Electron Microscopy (TEM)
 
​09:40 - 10:20
Lecture 6
Sergei Kopanchuk​
 
​Advance fluorescence microscopy methods to work with extracellular vesicles
 
​10:20 - 10:40
Tea & Coffee break 
 
​10:40 - 11:40
Workshop 8
Caius Miller
 
Advance fluorescence microscopy methods to work with extracellular vesicles
 
​11:40 - 12:20
Lecture 7
 
 
Lipidomics as a tool in EV research
Minna Holopainen
​12:20 - 13:20
Lunch
 
​13:20 - 15:00
Workshop 9 
Kasun Godakumara
 
​EV RNA cargo analysis and RNA extraction from EVs
​Subhashini Muhandiram
​15:00 - 15:20
Tea Break
 
​15:20 - 17:00
Workshop 10 
Kasun Godakumara
 
​Analysis of EV RNA quality and integrity
​​Subhashini Muhandiram
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​COMBIVET

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Institute of Veterinary Medicine and Animal Sciences

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​This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 857418.
  • Home
    • About us
    • Combivet Ethics Committee
    • Contact
  • Team
  • Research
    • ​Host-Pathogen Interactions and Disease Biomarkers Discovery >
      • Research project I
      • Research project II
    • Infertility and Subfertility >
      • Research project III
      • Research project IV
      • Research project V
    • ​Food, Feed & Health >
      • Research project VI
    • Non - Communicable Diseases and Cancer >
      • Research project VII
      • Research project VIII
  • Publications
  • NEWS & EVENTS
    • News
    • Seminars
    • Events >
      • Annual conference 2024
      • Research school 2024
      • Ethics debate 2023
      • Research school 2023
      • Annual conference 2023
      • Symposium 2023
      • Conference 2022
      • Summer School 2022
      • One Health Estonia 2022
      • CANLEISH/COMBIVET joint event 2022
  • University of Life Sciences
  • EUP AH&W