It is our great pleasure to invite you to second hands-on training school of "AU.873 Basics to work with extracellular vesicles (1ECTS)" jointly organized by COMBIVET ERA Chair of Comparative Medicine in the Institute of Veterinary Medicine and Animal Sciences of the Estonian University of Life Sciences, University of Tartu, Baltic Society of extracellular vesicles, University of Helsinki and University of Milan to be held from 5-7th of September 2023 in Tartu, Estonia.
Instructors
Program
Methods of EV Isolation
09:00 - 09:20 |
Welcoming and Introduction |
Alireza Fazeli |
09:20 - 10:05 |
Lecture 1 |
Kasun Godakumara |
A guide to working with extracellular vesicles: minimal experimental and reporting requirements |
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10:05 - 10:25 |
Tea & Coffee break |
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10:25 - 11:10 |
Lecture 2 |
Qurat Ul Ain Reshi |
Methods for EV purification |
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11:10 - 12:10 |
Student 3-minute presentations |
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12:10 - 13:10 |
Lunch |
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13:10 - 15:10 |
Workshop 1 |
Getnet Midekessa |
Introduction to Tangential Flow Filtration (TFF) |
Gayandi Ekanayake |
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15:10 - 15:30 |
Tea & Coffee break |
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15:30 - 17:00 |
Workshop 2 |
Qurat Ul Ain Reshi |
Size exclusion chromatography using manually packed chromatography column |
Madhusha Prasadani |
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Signe Parts |
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EV characterization and validation methods
09:00 - 09:40 |
Lecture 3 |
Sascha Raschke |
Introduction to Zeta view Nano particle tracking analyser |
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09:40 - 10:20 |
Lecture 4 |
Olavi Reinsalu |
Flow Cytometric analysis of EVs |
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10:20 - 10:40 |
Tea & Coffee break |
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10:40 - 11:10 |
Student 3-minute presentations |
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11:10 - 12:20 |
Workshop 3 |
Getnet Midekessa |
EV size and concentration measurements with NTA |
Chanaka Premathilaka Omid Mousavi |
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12:20 - 13:20 |
Lunch |
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13:20 - 14:20 |
Workshop 4 |
Getnet Midekessa |
Fluoroscence NTA |
Chanaka Premathilaka Omid Mousavi |
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14:20 - 15:20 |
Workshop 5 |
Getnet Midekessa |
EV zeta-potential measurements with NTA |
Chanaka Premathilaka Omid Mousavi |
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15:20 - 15:40 |
Tea & Coffee break |
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15:40 - 17:10 |
Workshop 6 |
Johanna Piibor |
NTA data analysis |
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17:10 - 17:50 |
Workshop 7 |
Olavi Reinsalu |
Flow Cytometric analysis of EVs |
Dulmini Nanayakkara |
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19:00 onwards |
Dinner |
Only with invitation |
EV validation methods and further applications
09:00 - 09:40 |
Lecture 5 |
Aneta Andronowska |
Transmission Electron Microscopy (TEM) |
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09:40 - 10:20 |
Lecture 6 |
Sergei Kopanchuk |
Advance fluorescence microscopy methods to work with extracellular vesicles |
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10:20 - 10:40 |
Tea & Coffee break |
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10:40 - 11:40 |
Workshop 8 |
Caius Miller |
Advance fluorescence microscopy methods to work with extracellular vesicles |
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11:40 - 12:20 |
Lecture 7 |
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Lipidomics as a tool in EV research |
Minna Holopainen |
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12:20 - 13:20 |
Lunch |
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13:20 - 15:00 |
Workshop 9 |
Kasun Godakumara |
EV RNA cargo analysis and RNA extraction from EVs |
Subhashini Muhandiram |
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15:00 - 15:20 |
Tea Break |
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15:20 - 17:00 |
Workshop 10 |
Kasun Godakumara |
Analysis of EV RNA quality and integrity |
Subhashini Muhandiram |
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