It is our great pleasure to invite you to the seventh hands-on training school of "Basics to work with extracellular vesicles" jointly organized by COMBIVET ERA Chair of Comparative Medicine in the Institute of Veterinary Medicine and Animal Sciences of the Estonian University of Life Sciences, OH-Boost, University of Tartu, Baltic Society of extracellular vesicles, University of Helsinki and University of Milan to be held from 21-23th of May 2024 in Tartu, Estonia.
Instructors
Program
Methods of EV Isolation
09:00 - 09:20 |
Welcoming and Introduction |
Alireza Fazeli |
09:20 - 10:05 |
Lecture 1 |
Subhashini Muhandiram |
A guide to working with extracellular vesicles: minimal experimental and reporting requirements |
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10:05 - 10:25 |
Tea & Coffee break |
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10:25 - 11:10 |
Lecture 2 |
Qurat Ul Ain Reshi |
Methods for EV purification |
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11:10 - 12:10 |
Student 3-minute presentations (Part 1) |
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12:10 - 13:10 |
Lunch |
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13:10 - 15:10 |
Workshop 1 |
Getnet Midekessa |
Introduction to Tangential Flow Filtration (TFF) |
Gayandi Ekanayake |
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15:10 - 15:30 |
Tea & Coffee break |
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15:30 - 17:00 |
Workshop 2 |
Signe Parts |
Size exclusion chromatography using manually packed chromatography column |
Madhusha Prasadani |
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EV characterization and validation methods
09:00 - 09:40 |
Lecture 3 |
Sascha Raschke |
Introduction to Zeta View Nano particle tracking analyser |
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09:40 - 10:20 |
Lecture 4 |
Sergei Kopanchuk |
Advanced microscopy to work with extracellular vesicles |
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10:20 - 10:40 |
Tea & Coffee break |
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10:40 - 11:10 |
Student 3-minute presentations (Part 2) |
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11:10 - 12:20 |
Workshop 3 |
Chanaka Premathilaka |
EV size and concentration measurements with NTA |
Omid Mousavi |
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12:20 - 13:20 |
Lunch |
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13:20 - 14:20 |
Workshop 4 |
Chanaka Premathilaka |
Fluoroscence NTA |
Omid Mousavi |
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14:20 - 15:20 |
Workshop 5 |
Chanaka Premathilaka |
EV zeta-potential measurements with NTA |
Omid Mousavi |
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15:20 - 15:40 |
Tea & Coffee break |
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15:40 - 16:45 |
Workshop 6 |
Madhusha Prasadini |
NTA data analysis |
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16:45 - 17:50 |
Workshop 7 |
Sergei Kopanchuk |
Advanced microscopy to work with extracellular vesicles |
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19:00 onwards |
Dinner |
Only with invitation |
EV validation methods and further applications
09:00 - 10:00 |
Lecture 5 |
Aneta Andronowska |
Transmission Electron Microscopy (TEM) |
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10:00 - 10:50 |
Lecture 6 (Virtual) |
Harinda Rajapaksha |
Introduction to Lipidomics |
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10:50 - 11:10 |
Tea & Coffee break |
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11:10 - 11:45 |
Workshop 8 |
Harinda Rajapaksha |
Introduction to EV Proteomics |
Subhashini Muhandiram Suranga Kodithuwakku |
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11:45 - 12:20 |
Workshop 9 (Part 1) |
Kasun Godakumara |
EV RNA cargo analysis and RNA extraction from EVs |
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12:20 - 13:20 |
Lunch |
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13:20 - 15:00 |
Workshop 9 (Part 2) |
Kasun Godakumara |
EV RNA cargo analysis and RNA extraction from EVs |
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15:00 - 15:20 |
Tea Break |
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15:20 - 17:00 |
Workshop 10 |
Kasun Godakumara |
EV RNA quality of and integrity analysis |
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17:00-17:30 |
Final student Evaluation |
Evaluation Committee |
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