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COMBIVET AND BALTIC SOCIETY OF EXTRACELLULAR VESICLES JOINT 

SUMMER SCHOOL 2022

COMBIVET AND BSEV JOINT SUMMER SCHOOL 2022

Basics to work with Extracellular Vesicles

The fifth research school organized by the Combivet Research group of The Estonian university of Life Sciences will take place from 27th of September to 29th of September​.

​In this exclusive summer school, the students will get hands on experience in the basics of EV isolation, characterization and the related data analysis from a panel of international experts with years of experience handling EVs. A series of lectures covering the required theoretical knowledge, best practices, tips and tricks, troubleshooting and adherence to the standards set by the International Society for Extracellular vesicles (ISEV) would also be offered. Latest developments in the field such as mass production of EVs will be thoroughly discussed along with the basics such as size exclusion chromatography, tangential flow filtration, ultracentrifugation, nanoparticle tracing analysis, flow cytometry for EVs, EV RNA extraction and characterization. The school is well suited for EV researchers who require a firm foundation in basics to begin their journey into the EV world.
Registration is closed!

AGENDA

  • Day 1
  • Day 2
  • Day 3
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​Methods of EV isolation - 27.09.2022

09:00 - 09:20
​  Welcoming and Introduction
​Alireza Fazeli

09:20 - 10:05
​Lecture 1
​A guide to working with extracellular vesicles:
​minimal experimental and reporting requirements
​Kasun Godakumara

10:05 - 10:25
​Tea & Coffee break

10:25 - 11:10
​Lecture 2
​Methods for EV purification 
​Qurat Ul Ain Reshi

11:10 - 12:10
Student 3-minute presentations (Part 1)

12:10 - 13:10
​Lunch

13:10 - 15:10
Workshop 1​
Introduction to Tangential Flow Filtration (TFF)
​Getnet Midekessa, Chanaka Premathilaka, Omid Mousavi  

15:10 - 15:30
Tea & Coffee break

15:30 - 17:30
Workshop 2
EV purification from follicular fluid samples:
​Size Exclusion chromatography 
​Qurat Ul Ain Reshi, Gayandi Ekanayake, Madhusha Prasadani

Summer School program
File Size: 454 kb
File Type: pdf
Download File

EV validation methods Nanoparticle​ - 28.09.2022

09:00 - 09:40
Lecture 3
​Introduction to Zetaview NTA
​Getnet Midekessa 

09:40 - 10:20
Lecture 4
Flow Cytometric analysis of Evs
​Olavi Reinsalu

10:20 - 10:40
Tea & Coffee break

10:40 - 11:20
Lecture 5
Lecture on Nano FCM
Rob Tempest

11:20 - 12:50
​Workshop 3
EV size and concentration measurements with NTA
​Getnet Midekessa, Chanaka Premathilaka,
​Omid Mousavi  

12:50 - 13:40
Lunch

13:40 - 14:40
Workshop 4
​Fluorescence NTA
​Getnet Midekessa, Chanaka Premathilaka,
​Omid Mousavi  

14:40 - 15:40
Workshop 5
​EV zeta-potential measurements with NTA 
​Getnet Midekessa, Chanaka Premathilaka,
​Omid Mousavi  

15:40 - 16:00
Tea & Coffee break

16:00 - 17:30
Workshop 6
​NTA data analysis 
​Johanna Piibor

17:30 - 18:00
Workshop 7
Flow Cytometric analysis of Evs
​Olavi Reinsalu

19:00
Gala Dinner

summer_school_2022_program.pdf
File Size: 454 kb
File Type: pdf
Download File

EV validation methods and further applications- 29.09.2022

09:00 - 09:40
Lecture 6
Transmission Electron Microscopy (TEM)
​Aneta Andronowska

09:40 - 10:20
Lecture 7
Mass spectrometry proteomics to interrogate extracellular vesicles
​Mandy Peffers

10:20 - 10:40
Tea & Coffee break

10:40 - 11:20
Lecture 8
Mass production of Evs
​Augustas Pivoriūnas

​11:20 - 12:20
Student 3-minute presentations (Part 2)

12:20 - 13:20
Workshop 8 
Nano FCM Demonstration
​Rob Tempest

13:20 - 14:20
Lunch

14:20 - 15:40
Workshop 9
​(part 1)
RNA extraction from Evs
​Kasun Godakumara and
​Subhashini Muhandiram

15:40 - 16:00
Tea & Coffee break

16:00 - 18:00
Workshop 9
​(part 2)
RNA extraction from Evs
Kasun Godakumara and
​Subhashini Muhandiram

Summer School program
File Size: 454 kb
File Type: pdf
Download File

TRAVEL INFORMATION

  • Transportation
  • Accommodation
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Traveling by plane? 
Estonian airport for international flights is located in Tallinn.

How can I get from Tallinn to Tartu?
A direct bus comes from Tallinn airport to Tartu. Bus tickets are limited and seats can be sold out when traveling last minute. To buy tickets in advance visit the Estonian official national bus ticket office website ​https://www.tpilet.ee/en/.​
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Transportation in Tartu?
Summer School will take place in Ravila 14b, Tartu, 50411. Google Maps directions are very helpful for reaching the destination. You can travel by taxi/Bolt, bus, bike or by foot. 

Tartu bus ticket information can be found on https://www.tartu.ee/en/inner-city-bus-transportation#ticket-prices. Tartu bus card retailers are found here: https://tartu.pilet.ee/pages/retailers and to load money or daily ticket on the card visit tartu.pilet.ee/buy.

To travel by bike you can rent a Smart Bike. The Smart Bike docks are right next to the Ravila 14b building. For more information visit https://ratas.tartu.ee/. ​
Accommodation can be booked through AirBnB  and Booking.com. 

Our recommendations for a pleasant stay in Tartu city centre:
Hotels: 
VSpa Hotel
Hotel Lydia
Hotel Tartu
Dorpat Hotel
​

Hostels: 
Hektor design hostel 
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​COMBIVET

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ADDRESS
Estonian University of Life Sciences
Institute of Veterinary Medicine and Animal Sciences

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Kreutzwaldi 62,
Tartu 51006,
tel/fax 7313 706
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​This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 857418.
  • Home
    • About us
    • Combivet Ethics Committee
    • Contact
  • Team
  • Research
    • ​Host-Pathogen Interactions and Disease Biomarkers Discovery >
      • Research project I
      • Research project II
    • Infertility and Subfertility >
      • Research project III
      • Research project IV
      • Research project V
    • ​Food, Feed & Health >
      • Research project VI
    • Non - Communicable Diseases and Cancer >
      • Research project VII
      • Research project VIII
  • Publications
  • NEWS & EVENTS
    • News
    • Seminars
    • Events >
      • Annual conference 2024
      • Research school 2024
      • Ethics debate 2023
      • Research school 2023
      • Annual conference 2023
      • Symposium 2023
      • Conference 2022
      • Summer School 2022
      • One Health Estonia 2022
      • CANLEISH/COMBIVET joint event 2022
  • University of Life Sciences
  • EUP AH&W