COMBIVET AND BSEV JOINT SUMMER SCHOOL 2022
Basics to work with Extracellular Vesicles
The fifth research school organized by the Combivet Research group of The Estonian university of Life Sciences will take place from 27th of September to 29th of September.
In this exclusive summer school, the students will get hands on experience in the basics of EV isolation, characterization and the related data analysis from a panel of international experts with years of experience handling EVs. A series of lectures covering the required theoretical knowledge, best practices, tips and tricks, troubleshooting and adherence to the standards set by the International Society for Extracellular vesicles (ISEV) would also be offered. Latest developments in the field such as mass production of EVs will be thoroughly discussed along with the basics such as size exclusion chromatography, tangential flow filtration, ultracentrifugation, nanoparticle tracing analysis, flow cytometry for EVs, EV RNA extraction and characterization. The school is well suited for EV researchers who require a firm foundation in basics to begin their journey into the EV world.
Registration is closed!
In this exclusive summer school, the students will get hands on experience in the basics of EV isolation, characterization and the related data analysis from a panel of international experts with years of experience handling EVs. A series of lectures covering the required theoretical knowledge, best practices, tips and tricks, troubleshooting and adherence to the standards set by the International Society for Extracellular vesicles (ISEV) would also be offered. Latest developments in the field such as mass production of EVs will be thoroughly discussed along with the basics such as size exclusion chromatography, tangential flow filtration, ultracentrifugation, nanoparticle tracing analysis, flow cytometry for EVs, EV RNA extraction and characterization. The school is well suited for EV researchers who require a firm foundation in basics to begin their journey into the EV world.
Registration is closed!
AGENDA
Methods of EV isolation - 27.09.2022
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09:00 - 09:20
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Welcoming and Introduction
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Alireza Fazeli
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09:20 - 10:05
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Lecture 1
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A guide to working with extracellular vesicles:
minimal experimental and reporting requirements |
Kasun Godakumara
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10:05 - 10:25
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Tea & Coffee break
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10:25 - 11:10
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Lecture 2
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Methods for EV purification
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Qurat Ul Ain Reshi
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11:10 - 12:10
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Student 3-minute presentations (Part 1)
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12:10 - 13:10
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Lunch
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13:10 - 15:10
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Workshop 1
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Introduction to Tangential Flow Filtration (TFF)
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Getnet Midekessa, Chanaka Premathilaka, Omid Mousavi
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15:10 - 15:30
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Tea & Coffee break
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15:30 - 17:30
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Workshop 2
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EV purification from follicular fluid samples:
Size Exclusion chromatography |
Qurat Ul Ain Reshi, Gayandi Ekanayake, Madhusha Prasadani
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| Summer School program |
EV validation methods Nanoparticle - 28.09.2022
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09:00 - 09:40
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Lecture 3
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Introduction to Zetaview NTA
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Getnet Midekessa
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09:40 - 10:20
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Lecture 4
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Flow Cytometric analysis of Evs
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Olavi Reinsalu
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10:20 - 10:40
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Tea & Coffee break
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10:40 - 11:20
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Lecture 5
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Lecture on Nano FCM
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Rob Tempest
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11:20 - 12:50
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Workshop 3
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EV size and concentration measurements with NTA
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Getnet Midekessa, Chanaka Premathilaka,
Omid Mousavi |
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12:50 - 13:40
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Lunch
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13:40 - 14:40
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Workshop 4
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Fluorescence NTA
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Getnet Midekessa, Chanaka Premathilaka,
Omid Mousavi |
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14:40 - 15:40
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Workshop 5
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EV zeta-potential measurements with NTA
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Getnet Midekessa, Chanaka Premathilaka,
Omid Mousavi |
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15:40 - 16:00
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Tea & Coffee break
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16:00 - 17:30
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Workshop 6
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NTA data analysis
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Johanna Piibor
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17:30 - 18:00
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Workshop 7
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Flow Cytometric analysis of Evs
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Olavi Reinsalu
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19:00
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Gala Dinner
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| summer_school_2022_program.pdf |
EV validation methods and further applications- 29.09.2022
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09:00 - 09:40
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Lecture 6
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Transmission Electron Microscopy (TEM)
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Aneta Andronowska
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09:40 - 10:20
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Lecture 7
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Mass spectrometry proteomics to interrogate extracellular vesicles
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Mandy Peffers
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10:20 - 10:40
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Tea & Coffee break
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10:40 - 11:20
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Lecture 8
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Mass production of Evs
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Augustas Pivoriūnas
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11:20 - 12:20
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Student 3-minute presentations (Part 2)
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12:20 - 13:20
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Workshop 8
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Nano FCM Demonstration
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Rob Tempest
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13:20 - 14:20
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Lunch
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14:20 - 15:40
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Workshop 9
(part 1) |
RNA extraction from Evs
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Kasun Godakumara and
Subhashini Muhandiram |
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15:40 - 16:00
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Tea & Coffee break
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16:00 - 18:00
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Workshop 9
(part 2) |
RNA extraction from Evs
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Kasun Godakumara and
Subhashini Muhandiram |
| Summer School program |
TRAVEL INFORMATION
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Traveling by plane?
Estonian airport for international flights is located in Tallinn. How can I get from Tallinn to Tartu? A direct bus comes from Tallinn airport to Tartu. Bus tickets are limited and seats can be sold out when traveling last minute. To buy tickets in advance visit the Estonian official national bus ticket office website https://www.tpilet.ee/en/. |
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Transportation in Tartu?
Summer School will take place in Ravila 14b, Tartu, 50411. Google Maps directions are very helpful for reaching the destination. You can travel by taxi/Bolt, bus, bike or by foot.
Tartu bus ticket information can be found on https://www.tartu.ee/en/inner-city-bus-transportation#ticket-prices. Tartu bus card retailers are found here: https://tartu.pilet.ee/pages/retailers and to load money or daily ticket on the card visit tartu.pilet.ee/buy.
To travel by bike you can rent a Smart Bike. The Smart Bike docks are right next to the Ravila 14b building. For more information visit https://ratas.tartu.ee/.
Summer School will take place in Ravila 14b, Tartu, 50411. Google Maps directions are very helpful for reaching the destination. You can travel by taxi/Bolt, bus, bike or by foot.
Tartu bus ticket information can be found on https://www.tartu.ee/en/inner-city-bus-transportation#ticket-prices. Tartu bus card retailers are found here: https://tartu.pilet.ee/pages/retailers and to load money or daily ticket on the card visit tartu.pilet.ee/buy.
To travel by bike you can rent a Smart Bike. The Smart Bike docks are right next to the Ravila 14b building. For more information visit https://ratas.tartu.ee/.
Accommodation can be booked through AirBnB and Booking.com.
Our recommendations for a pleasant stay in Tartu city centre:
Hotels:
VSpa Hotel
Hotel Lydia
Hotel Tartu
Dorpat Hotel
Hostels:
Hektor design hostel
Our recommendations for a pleasant stay in Tartu city centre:
Hotels:
VSpa Hotel
Hotel Lydia
Hotel Tartu
Dorpat Hotel
Hostels:
Hektor design hostel
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